University of Twente Student Theses
Cell-instructive membrane biomaterial for non-union bone healing
Rojas Moreno, Efrén Miguel (2024) Cell-instructive membrane biomaterial for non-union bone healing.
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| Abstract: | Enhancing the healing of non-union bone fractures is a critical challenge in orthopaedic medicine, necessitating the development of advanced biomaterials. This study investigates the efficacy of P15 peptide-functionalised polyethylene terephthalate (PET) membranes in promoting osteogenic differentiation and bone regeneration. The PET membranes were treated to attach P15 peptides using oxygen plasma, NaOH, and EDC/NHS coupling, with successful functionalisation confirmed via contact angle measurements. Human osteoblast cells (hOBs) were then cultured on native, Collagen I-coated, and P15-treated membranes, and their interactions were analysed through immunofluorescence staining and confocal microscopy to assess integrin expression, focal adhesion formation, and cell morphology. Results demonstrated that P15-functionalised PET membranes indicate potential for good cell adhesion, integrin localisation, and focal adhesion formation, suggesting a conducive environment for osteogenic activity. These findings indicate that P15-functionalised PET membranes hold promise for enhancing bone regeneration in non-union fractures, supporting their potential application in clinical orthopaedic treatments. |
| Item Type: | Essay (Bachelor) |
| Faculty: | EEMCS: Electrical Engineering, Mathematics and Computer Science |
| Subject: | 35 chemistry, 42 biology, 44 medicine |
| Programme: | Technology and Liberal Arts & Sciences BSc (50427) |
| Link to this item: | https://purl.utwente.nl/essays/100394 |
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