University of Twente Student Theses
Model-based wavefront shaping to improve imaging in lung-on-a-chip devices
Gorsel, Merle van (2021) Model-based wavefront shaping to improve imaging in lung-on-a-chip devices.
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| Abstract: | Model-based wavefront shaping is investigated as a method to improve the focus inside two lung-on-a-chip samples. A two-photon microscope is used to obtain a stack of images of the interface, which are used to reconstruct a digital model of the refractive index distribution of the sample. This digital refractive index model is used to perform a virtual phase conjugation experiment. We simulate the propagation of light from a virtual point source through the refractive index model of the sample to find the aberrated field. If the phase conjugate of the aberrated field is propagated back through the sample, the light forms a focus at the position of the virtual point source due to the time-reversal nature of light. By making a simulation of the virtual phase conjugation experiment, we calculated a theoretical maximum increase in intensity of 3.2x. In a proof of concept experiment, we achieved a 1.8x increase of intensity in a bulk of fluorescein inside a PDMS sample. Further, using a collagen matrix sample with epithelial cells, we reconstructed the refractive index distribution without labeling, by utilizing collagen's second-harmonic properties. Simulations predict a potential 169 times increase in intensity, though this hasn't been experimentally confirmed yet. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 33 physics |
| Programme: | Applied Physics MSc (60436) |
| Link to this item: | https://purl.utwente.nl/essays/101867 |
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