University of Twente Student Theses
Characterizing α-Synuclein Polymorphs Through AFM, SAA, Spectrophotometry and Digestion Assay
Chen, W. (2024) Characterizing α-Synuclein Polymorphs Through AFM, SAA, Spectrophotometry and Digestion Assay.
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| Abstract: | SAAs have made it possible to detect PD by being able to identify α-synuclein aggregations. Currently, researchers are working on finding out how polymorphs affect SAAs, as well as on how they affect synucleinopathies. This report will emphasize on finding discriminatory properties of different strains first and will serve as a stepping stone for the previous mentioned research purposes. To begin with, according the amplification rates were significantly different between the polymorphs. In addition, through AFM it has been found that although height does not differ among the strains, periodicity does. Furthermore, some strains produced unique spectra in the spectrophotometer when using the dyes ThT, Amy, ANS and NR, implying differences in how well each strain binds to the dye, which is influenced by the structure of the strain. Lastly, no conclusion could be drawn from the results of the proteinase K digestion, as the experiment was not successfully executed. Overall, some unique properties of strains have been found through these methods. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 33 physics, 35 chemistry, 42 biology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/102089 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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