University of Twente Student Theses
The osteogenic capacity of a novel bone graft substitute
Blokhuis, BSc J.T. (2024) The osteogenic capacity of a novel bone graft substitute.
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| Abstract: | Purpose: Iliac crest bone graft is considered the gold standard for bone grafting. However, this method has its drawbacks, an alternative is therefore desired. Recently, a novel surgical suction filter device was introduced to the market which can generate a bone graft substitute. The osteogenic capacity of this novel bone graft has not yet been determined. Materials and Methods: BoneFlo (BF) samples were obtained during non-union surgery. The BF was loaded with a collagen based β-TCP containing foam sponge and fitted to the surgical suction device upon opening the intramedullary canal. As a control for the BoneFlo, whole blood was used. Samples were compared to cells from the bone marrow (BMAC) and to RIA samples, which is another alternative bone graft. On day 0, BF samples were analysed for expression of osteogenic marker genes and cell composition was investigated. Additionally, samples were cultured and analysed on day 7, 14 and 21 for ALP activity, matrix mineralization and expression of osteogenic marker genes. Results: In total, 13 BF, 3 BMAC and 1 RIA sample were used for the experiments. The BF samples showed distinct differences in comparison to the control samples in gene expression and ALP activity. In addition, the dynamic expression profile of the BF samples is comparable to the expression profile of the BMAC and RIA group. Conclusion: This study shows that the BoneFlo can create an osteogenic bone substitute as it enhances the expression of osteogenic marker genes and has a higher ALP activity in comparison to the control groups. It may therefore be a promising tool to generate a less invasive bone graft substitute. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/102307 |
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