University of Twente Student Theses
Detection function of cartilage VHH on synovial fluid antigen detection for OA diagnosis
Hovius, Nile (2024) Detection function of cartilage VHH on synovial fluid antigen detection for OA diagnosis.
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| Abstract: | Osteoarthritis (OA) is a degenerative disease of the joint, most common in the knee. OA occurs in more than 500 million patients worldwide, and is one of the leading causes of pain and disability in the elderly. Currently, OA can not be cured, and the treatment that does exist has not been shown to modify the disease, at best providing pain relief at the cost of severe side effects. Diagnosis is often too late, at which point irreversible damage has already been done. Biochemical methods using biomarkers to predict, diagnose, or monitor disease have long been a point of interest of OA researchers. Biomarkers may be able to diagnose OA in a very early stage. However, no such biomarker is currently accepted as characteristic of OA. VHH antibodies may play a large role in OA biomarker discovery. In this study, ELISA is performed on synovial fluid samples using VHHs selected from phage-display biopanning on decellularized cartilage, in order to validate whether or not these cartilage specific VHH can also accurately detect antigens in synovial fluid. Furthermore, the synovial fluid samples donors have been treated with Celecoxib or Naproxen, or received no treatment prior to knee removal surgery, and sample retrieval. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/102810 |
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