University of Twente Student Theses
Implementation of an autocatalytic signal amplifying protocol in digital droplet assays
Lampe, Kevin (2025) Implementation of an autocatalytic signal amplifying protocol in digital droplet assays.
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| Abstract: | This thesis is a first attempt to implement AutoCAR-1 in a digital droplet CRISPR assay, a novel attempt to lower the limit of detection (LoD) in Cas-based DNA detection. AutoCAR-1 amplifies the signal instead of the target by introducing a secondary target, a linearized circular mediator. Difficulties in synthesizing the circular mediator meant that experimental work shifted towards troubleshooting the synthesis protocol. No conclusive solution was found, so we conclude with recommendations for further research into mediator synthesis. |
| Item Type: | Essay (Bachelor) |
| Faculty: | EEMCS: Electrical Engineering, Mathematics and Computer Science |
| Subject: | 42 biology |
| Programme: | Technology and Liberal Arts & Sciences BSc (50427) |
| Link to this item: | https://purl.utwente.nl/essays/104952 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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