University of Twente Student Theses
Prototype of a Multiplexed Microfluidic Device For Point-of-Care Nanopore-based Diagnostics
Katklian, J. (2025) Prototype of a Multiplexed Microfluidic Device For Point-of-Care Nanopore-based Diagnostics.
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| Full Text Status: | Access to this publication is restricted |
| Embargo date: | 24 April 2029 |
| Abstract: | With the growing demand for precision medicine and portable diagnostic tools, nanopore-based devices offer a promising solution. This work presents a prototype 12x multiplexed microfluidic device designed for individual nanopore recordings and a proof of concept for frequency multiplexing of Bilayer Lipid Membranes (BLMs). Channels were fabricated by milling a PMMA substrate, achieving a width and height of 1 mm. A Teflon film, punctured with a 300-micron needle, served as the foundation for BLM formation. BLMs were formed by applying n-Hexadecane and DPhPC lipids onto the aperture and flowing buffer back and forth to induce a shear-thinning effect. By incorporating resistors at channel junctions, individual nanopore measurements remain stable even if neighboring BLMs rupture. Device validation was demonstrated using substrate binding domain 2 (SBD2) measurements in a type I cytolysin A (ClyA) nanopore, where both open configurations of SBD2 were distinguished via patch-clamp. Frequency multiplexing was explored to enable the formation of more than two BLMs per channel without introducing sneak paths. Electrochemical impedance spectroscopy (EIS) revealed shifts in BLM cut-off frequency driven by series resistance and buffer molarity variations, indicating selective measurement based on input frequencies. These results highlight frequency multiplexing as a promising strategy to increase device throughput. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 30 exact sciences in general, 42 biology, 53 electrotechnology |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/106218 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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