University of Twente Student Theses
Seeda Amplification Assay for alphaSynuclein Fibrils: Employing Single Particle Imaging Towards a Quantitative Evaluation of Seed Amplification Assays
Kötterheinrich, Phil (2025) Seeda Amplification Assay for alphaSynuclein Fibrils: Employing Single Particle Imaging Towards a Quantitative Evaluation of Seed Amplification Assays.
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| Abstract: | The early detection of Parkions Disease[PD] is important for the treatment of patients, but also to help researcherss understand the mechanisms of PD. Amyloid aSynuclein (aSyn) fibrils have recently identified as the first non post-mortem biomarker for PD. THe fibrils are not only found in the brain but also the cerebral spinal fluid (CSF), blood or even in the olfactory mucosa. Seed Amplification Assays (SAA) have ben developed for the detection of aSyn. THe SAAs provide a yes/no answer whether amyloid fibrils are present in a sample or not, but struggle to provide a quantiatvie result. The goal of this thesis was to develop a proof-of-principle protocol for a quantitative SAA based on Single Particle Imagaing (SPI). The adaptadion process of the "standard" RT-QuiC process is shown. And the challenges of the use of SPI for the imaging of amyloid fibrils are presented, Iterative solutions to upcoming problems were discussed and in the end a protocol for a quantitative SPI based SAA is presented. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 44 medicine |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/106303 |
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