University of Twente Student Theses
Towards the Quantification of Fluorescence Angiography for Perfusion Assessment
Eikholt, R.R. (2025) Towards the Quantification of Fluorescence Angiography for Perfusion Assessment.
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| Full Text Status: | Access to this publication is restricted |
| Embargo date: | 7 May 2026 |
| Abstract: | Purpose: Fluorescence angiography (FA) with indocyanine green (ICG) is widely used to assess intestinal perfusion and reduce anastomotic leakage (AL). However, its interpretation remains qualitative and patient-specific due to variability in arterial input functions (AIFs). This thesis aims to enable quantitative FA by implementing pulse oximeter-based AIF estimation and validating a correction algorithm in phantom experiments. Methods: Two flow phantoms were developed. The first simulated microcirculation using glass beads to generate time-intensity curves (TICs) under varying ICG injection parameters, flow rates, and bead packing densities. The second mimicked pulsatile flow to estimate AIFs using a commercial pulse oximeter. A deconvolution algorithm was developed to correct TICs using the measured AIFs. Results: The microcirculation phantom produced TICs reflecting changes in perfusion and injection parameters. The pulse oximeter captured dynamic ICG signals, but real-time AIF estimation was limited by hardware/software constraints. Deconvolution improved TIC comparability and isolated tissue-specific responses. Conclusions: This study demonstrates the feasibility of correcting FA measurements using non-invasively estimated AIFs. It also identifies key modifications required to adapt pulse oximeters for clinical AIF estimation, offering a step toward quantitative FA in surgical settings. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 01 general works |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/106324 |
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