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Development of a Joint-on-Chip platform
Kramer, Marlize (2025) Development of a Joint-on-Chip platform.
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| Full Text Status: | Access to this publication is restricted |
| Embargo date: | 3 July 2027 |
| Abstract: | Multi-Organ-on-Chips (multi-OoCs) are promising in vitro models to investigate disease mechanisms and potential drugs. However, the translation of this model in a multiplexed multi-OoC platform can become challenging. These platforms facilitate actuation of the distinct models and real-time measurements. Modular platforms have gained much interest by the scientific community, due to their high potential to accelerate the integration of multi-OoC models on platforms. The modularity offers flexibility in design adaptions, and integration of components from multiple suppliers. However, current available platforms are not interoperable, leading to a fragmented ecosystem. To address the lack of standardisation, the ISO standard 22916:2022 has been developed to improve the interoperability of microfluidic components. Furthermore, the concept of a Translational Organ-on-chip Platform (TOP) has been developed to facilitate biomedical research on an interoperable, open, modular platform. However, the design process of integrating a multiplexed multi-OoC model on TOP is not yet standardised. In this master thesis, a knee joint disease model for arthritis is translated to a multi-OoC platform to standardise the design process of modular platforms. In this Joint-on-Chip (JoC) platform, a Cartilage-on-Chip and Synovium-on-Chip are co-cultured to realise chondro-synovial crosstalk in combination with pneumatic stimulation. A general design process has been formulated that includes automatic routing of the complex microfluidic and pneumatic networks. Quality control benchmarks are discussed to enhance the reproducibility of the platform fabrication. The JoC platform has shown its robustness in reproducibility and preliminary validation results demonstrate the potential in performance. However, the flow and mechanobiological stress on the platform are not yet well validated. After these validations, the JoC platform has great potential to enhance arthritis research. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 44 medicine, 50 technical science in general, 53 electrotechnology |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/107269 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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