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Designing a laser scanning confocal microscope: a new scan engine layout

Duinen, D. van (2013) Designing a laser scanning confocal microscope: a new scan engine layout.

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Abstract:Background: For several optical imaging systems a scan engine is used. The currently used scan engines use 2 mirrors and, although this makes the scanning fairly easy it also introduces a inherent error in the beam’s position. The targeted application for the newly designed scan engine is a laser scanning confocal microscope. Approach: The error introduced originates from the mirrors having different deflection origins. To displace one of the origins to coincide a third, correcting mirror can be introduced. However, when another, fourth mirror is introduced both origins can be controlled in space and hence a full 3D control of this point is obtained. Also the scan mirrors and their controls and feedback systems have been characterized. The system used is a “High speed Galvo System, AXJ-V20 close-loop scanner”. Yet before this the system was optimized as some settings were adjustable. Results: An expression for a pivot point was analytically found, as function of the angles of all four scan mirrors. The system seemed too coupled to solve analytically and hence a numerical approach using a covariant matrix adaptation evolution strategy was tried. This resulted in a set of angles for which the pivot point remained stationary. The system was optimized and its response for several frequencies and deflections were obtained. Conclusions: The newly designed scan system can be applied in a microscope or imaging system. The galvanometers have been characterized and thus is ready for operation.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:33 physics
Programme:Applied Physics BSc (56962)
Link to this item:http://purl.utwente.nl/essays/63632
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