University of Twente Student Theses
As of Friday, 8 August 2025, the current Student Theses repository is no longer available for thesis uploads. A new Student Theses repository will be available starting Friday, 15 August 2025.
Modeling of protein secretion by cells
Baumgartner, Wolfgang (2014) Modeling of protein secretion by cells.
|
PDF
1MB |
| Abstract: | Protein secretion by cells can be measured with surface plasmon resonance. However, based on the fact that the protein immobilized on the sensor surface and the produced protein are specific to each other, this method only measures the produced protein that binds to the surface. It was unknown beforehand how much protein doesn't reach the SPR sensor and stays in the bulk of the cell medium. To find out about this, a model was devised that sufficiently re ected the practical experiment and led to a simulation. From this simulation, it can be concluded that, under given circumstances, 99.1% of the total cell product binds to the surface. Therefore, single cell SPR measurements are an accurate way to determine cell production rates. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 42 biology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/66573 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
Show download statistics for this publication
Show download statistics for this publicationRepository Staff Only: item control page