In vitro studies of the interaction between α-synuclein and SNAREs

Siero, C.J. (2015) In vitro studies of the interaction between α-synuclein and SNAREs.

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Abstract:Previous studies have hinted at the role of α-synuclein on the SNARE mediated release of synaptic vesicles and one of these studies has shown an interaction between α-synuclein and one of these SNARes, synaptobrevin, No data is known about the strength of the interaction between α-synuclein and synaptobrevin or if any interaction takes place between α-synuclein and the other SNAREs SNAP25 and syntaxin. The SNAREs have been produced and purified untill the desired concentrations and purity of protein has been achieved. Attempts have been made to optimize the production of the however the requirements have only been met with the acquisition and production of new plasmid constructs of the desired SNAREs. The α-synuclein protein was shown to interact with all three of the SNAREs however the interaction between α-synuclein and synaptobrevin proved to be of higher affinity than the interaction of α-synuclein with SNAP25 or syntaxin. The interaction between α-synuclein and synaptobrevin at increasing Ca2+ concentration, increases the affinity between these proteins. Both the α-synuclein and the synaptobrevin interact with Ca2+-ions and upon monitoring the binding between these proteins in conditions of increasing Ca2+ concentration the dissociation constant drops.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology
Programme:Biomedical Engineering MSc (66226)
Link to this item:http://purl.utwente.nl/essays/68283
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