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µFlow : A compact fluorescence detection system that uses a DVD pickup for the optical detection of beads

Koster, M.J. (2011) µFlow : A compact fluorescence detection system that uses a DVD pickup for the optical detection of beads.

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Abstract:The fertility project started at BIOS, Lab on a Chip group, in 2007, aims at developing a high accuracy point of care semen analyzer that can be used at home, making the semen analysis more comfortable, easier, and cheaper. During a semen analysis the semen quality is to be determined by concentration, motility, morphology and vitality/viability of the sperm cells. At present the concentration and motility can be determined with a microfluidic chip using electrical conductivity measurements. To measure the remaining parameters, new detection methods may be developed and the first results thereof are described in this thesis. In this thesis, the use of fluorescence detection is described. Using a ‘Lab on a Chip’ and an optical pickup taken from a DVD player, a fluorescence detection apparatus, the ‘μflow’ has been designed and built. The optical pickup has a lot of integrated optical components that makes a compact, easy to use and cheap fluorescence detection system possible. A ‘Lab on a Chip’ consisting of three microfluidic channels and digital marks for position detection has been developed. Automatic guiding the optical pickup to find the three channels was successfully shown using –on chip- digital marks and an automatic focus system. The μflow fluorescence detection capabilities were tested using 6 μm and 2.5 μm fluorescent beads and real-time 2D visualisation of the beads in Labview was demonstrated. Beads were counted and the size, speed, transversal and vertical position of the individual beads in the fluidic channel were determined. The optical resolution is limited by the spot size of the laser and therefore only particles larger than 0.4 μm could be visualized. No experiments were performed using spermatozoa, but it is very likely that spermatozoa can be visualized with the same resolution as beads. If this succeeds for spermatozoa, the μflow would be able to determine the concentration and morphology parameters of the sperm cells (vitality/viability can be assessed using fluorescent dyes). The μflow was built in such a way that in a future project it may also be used for analyzing other biological relevant fluids such as blood.
Item Type:Essay (Master)
Faculty:EEMCS: Electrical Engineering, Mathematics and Computer Science
Subject:53 electrotechnology
Programme:Electrical Engineering MSc (60353)
Link to this item:http://purl.utwente.nl/essays/69783
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