University of Twente Student Theses


Novel markers for detection of CTCs without EpCAM expression

Hudepol, C.E. (2017) Novel markers for detection of CTCs without EpCAM expression.

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Abstract:Circulating tumour cells (CTCs) in the blood of a cancer patient can give information on the status of the cancer. Tumour cells that enter the blood stream can have different phenotypes. Epithelial cells express antigens that are mostly involved in cell adhesion and cell signalling. Mesenchymal cells on the other hand express antigens that are important for cell motility and movement. CTCs can express either one of the phenotypes, or reside in a state where antigens of both phenotypes are expressed. CTCs are currently isolated using the CELLSEARCH® system, which employs EpCAM and Cytokeratins to identify the tumour cells. However, since both antigens are characteristic for the epithelial phenotype, it could be that cells of the mesenchymal phenotype are lost. The purpose of this research is to give an indication of the role of epithelial-mesenchymal transition in the expression of different antigens, and to identify antigens that can be used next to EpCAM and Cytokeratins in the detection of CTCs. To reach this goal, immunostaining was optimised, and the mesenchymal antigens Vimentin and Cadherin 11 were tested on cell lines with varying EpCAM expression.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:30 exact sciences in general, 44 medicine
Programme:Biomedical Technology BSc (56226)
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