University of Twente Student Theses
Hydrogel-based 3D semi-circular microvascular bbb-on-chip with integrated sensors for barrier assessment through TEER measurements
Heus, L.E. de (2018) Hydrogel-based 3D semi-circular microvascular bbb-on-chip with integrated sensors for barrier assessment through TEER measurements.
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| Abstract: | Realistic models that optimally mimic the blood-brain barrier (BBB) are essential for researches regarding drug passage across the barrier that aim for developing adequate neuropharmaceuticals. Here, we propose a hydrogel-based 3D microvascular BBB-on-chip with integrated sensors for barrier function assessment through TEER. Hyaluronic-based hydrogels suitable for this application were identified by their ability to adopt channel features from a mould and subsequently seal on a chemically functionalized glass substrate. To investigate the suitability of the selected hydrogel for cell culture purposes, human cerebral endothelial cells (hCMEC/D3) were cultured on hyaluronic-based hydrogels complemented with gelatin to promote cell adhesion. Finally, the developed sensor comprised of electrodes incorporated in a glass slide was characterized using hydrogel-based electrolytes with increasing conductivity. During the scope of this thesis, first steps were made in achieving a hydrogel-based microfluidic chip with integrated sensors. Open channels could be fabricated in hyaluronic-based hydrogel, which proved suitable for endothelial cell culture. Moreover, preliminary tests with the electrode configurations showed its potential for ultimately TEER measurements on chip. Future research on channel sealing is vital for completing the device such that it facilitates cell culture and TEER measurements. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 42 biology, 44 medicine, 50 technical science in general, 53 electrotechnology |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/77162 |
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