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Micropatterns on Polyacrylamide Hydrogel for Controlled Cardiomyocyte Attachment using PLL-PEG and ECM Proteins

Dorenvanck, K.M. van (2019) Micropatterns on Polyacrylamide Hydrogel for Controlled Cardiomyocyte Attachment using PLL-PEG and ECM Proteins.

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Abstract:Poly-L-Lysine-graft-poly(ethylene-glycol) PLL-PEG and extracellular matrix (ECM) proteins can be used to create micropatterned areas for cardiomyocyte cell adhesion and surface passivation on polyacrylamide (PAA). This research endeavoured to optimize a protocol designed to create these cell micropatterns using several combinations of PLL-PEG and ECM cell adhesive proteins vitronectin, fibronectin, gelatin, and gelatin-methacrylate (gelMA). Vitronectin, fibronectin and gelatin were used as a coating, where gelMA was mixed in the PAA. The results for the cell micropatterns were inconsistent, with identical or similar methods giving either no, correct, or inverse micropatterns. The most consistent and correct micropatterns were found for the use of PLL-PEG and PAA-gelMA. The highest quality of micropatterns was found for PLL-PEG and gelatin on PAA, however, these patterns were inverse. Furthermore, the removal of excess PLL-PEG after coating was found to increase micropattern overall quality. Additionally, some minor protocol optimizations were made.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology, 44 medicine
Programme:Biomedical Engineering MSc (66226)
Link to this item:http://purl.utwente.nl/essays/79533
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