University of Twente Student Theses


Bioprintability of Dex-TA/HA-TA hydrogels with human iPS cells for cartilage tissue engineering

Nauta, L. (2019) Bioprintability of Dex-TA/HA-TA hydrogels with human iPS cells for cartilage tissue engineering.

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Abstract:Articular cartilage damage often leads to osteoarthritis, a major global cause of disability. Three-dimensional (3D) bioprinting holds large potential for cartilage tissue engineering, but there is a lack of suitable bioinks. In this study, the bioprintability of Dex-TA/HA-TA hydrogel with human induced pluripotent stem cells (iPSCs) was explored. Upregulation of ACAN expression in iPSCs was demonstrated for a variety of Dex-TA/HA-TA gel compositions. Enzymatic pre-crosslinking was successfully used to tune the viscosity of Dex-TA/HA-TA and create shear thinning bioinks. For the first time, printing and enzymatic post-crosslinking of such a bioink with iPSCs was demonstrated. However, sensitivity of the pre-crosslinking process yielded substantial variations in viscosity of the resulting bioink and was highly affected by the presence of iPSCs. In addition, pre-crosslinked bioinks show strong thixotropic behaviour including permanent viscosity loss, which could impair post-printing shape fidelity. In the future, a more robust 3D bioprinting platform for cartilage tissue engineering could be established by combining or replacing the enzymatic pre-crosslinking with another crosslinking mechanism, or increase the pre-printing viscosity using another approach.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:35 chemistry, 44 medicine, 51 materials science
Programme:Biomedical Engineering MSc (66226)
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