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Development of a myocardial infarction model by the design of a miniaturized 3D culture platform

Boonen, T.M.P.M. (2019) Development of a myocardial infarction model by the design of a miniaturized 3D culture platform.

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Abstract:Currently the leading cause of death in the western world is cardiovascular disease (CVD) and in the future the amount of people suffering from CVD is only projected to rise. However high-throughput disease models are lacking. Since the generation of the first induced pluripotent stem cells (iPSCs), these iPSCs have become of great interest in the field of drug testing and disease modelling. The big problem with cardiomyocytes derived from iPSCs (iPSC-CM) is that after differentiation these cells show an immature phenotype which manifests itself in an immature metabolism. Because of this immature metabolism they cannot yet be used in modeling CVD. During this project an attempt has been taken at maturing iPSC-CMs in two dimensional (2D) cell culture, by alteration of the carbon source available in the medium. Unfortunately no mature metabolism was achieved and that is why the focus shifted towards three dimensional (3D) culture possibilities. A 3D culture method of suspending a strip of cardiac cells between two flexible pillars called an engineered heart tissue (EHT) has been incorporated into a microfluidic platform. This platform, the microEHT array, allows for easy parallel culture of miniaturized EHTs for over two months.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:02 science and culture in general
Programme:Biomedical Engineering MSc (66226)
Link to this item:http://purl.utwente.nl/essays/79975
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