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In vitro modeling of cardiac arrhythmia : Geometrically constrained tissue culture and acoustic pacing with ultrasound

Bugter, Jeroen (2020) In vitro modeling of cardiac arrhythmia : Geometrically constrained tissue culture and acoustic pacing with ultrasound.

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Abstract:As cardiac arrhythmias are an ever increasing burden on health and healthcare costs, there is need for more reliable methods of treatment. Development of such treatments is hindered by the lack of reliable in vitro models for disease and drug research. This thesis consists of 2 reports of separate studies within the overarching context of in vitro modeling of cardiac arrhythmia. Specifically, a method was developed to study re-entry induced by a source-sink mismatch. Using geometrically constrained tissue culture, hESC-derived cardiac microtissues containing a source-sink mismatch were created, and the ideal culture conditions were optimized. Readout was performed by means of voltage optical mapping and calcium imaging. The second study involves the development of acoustic pacing of in vitro cardiomyocytes through ultrasonic pulses. Such a method could provide an alternative to the existing electrical and optogenetic pacing methods, which suffer from cytotoxicity and a lack of excitation depth respectively. Tissues made from hESC-derived cardiac cells were subjected to ultrasonic pulses of various frequencies and pulse durations. Various observed types of response were catalogued. While actual pacing was at best transiently achieved, the data obtained should provide a start for further developmentof this promising method.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology
Programme:Biomedical Engineering MSc (66226)
Link to this item:http://purl.utwente.nl/essays/81241
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