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Axial superresolution using nanophotonic manipulation: How accurate can we be?

Dinteren, R.A.J. van (2020) Axial superresolution using nanophotonic manipulation: How accurate can we be?

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Abstract:The nanoscale topography of the cell membrane is often overlooked. However, the deformation of the cell membrane plays a role in many processes. Current imaging methods struggle to measure nanoscale features on live cells. A novel approach has been developed using photonic emitter manipulation to achieve axial sub-20 nm resolution without directly interacting with the surface. Here we continue characterizing this approach. We observe that the feedback-mechanism can be influenced by the surface, hindering reproducibility. We calculate the axial resolution from experiment and simulation, displaying its distance-dependency and the working range of the technique. We show an undesirable change to the point spread function under effect of the emitter manipulation. The technique shows promise for axial superresolution imaging. However, there are fundamental obstacles that must be overcome in order to measure on live cells.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:33 physics, 42 biology
Programme:Biomedical Engineering MSc (66226)
Link to this item:http://purl.utwente.nl/essays/85106
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