University of Twente Student Theses


Modelling and Design of Multiplexed Organ-on-chip with Integrated TEER Sensing

Majumdar, Arghya (2020) Modelling and Design of Multiplexed Organ-on-chip with Integrated TEER Sensing.

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Abstract:One of the parameters used in Organ-on-chip platforms to monitor barrier formation and observe the tight junction integrity of the cell cultures is TEER or Transendothelial Electrical Resistance. In this assignment, an Organ-on-chip (OoC) setup was designed with integrated TEER sensing capabilities. The electrode system to be implemented in the chip was modelled and simulation studies were conducted on COMSOL Multiphysics® v5.4. Measurements of TEER in Organ-on-chips often provide erratic results and so sensitivity distribution studies were done to understand the performance of the design. Two different configurations were used to define the electrodes as current-carrying and pick-up electrodes. It was found that in case of both configurations, the normalized sensitivity (Sn) is less uniform for lower TEER values. However, the distribution approaches uniformity (Sn=1) with an increase in TEER value. The first configuration was found to be less erroneous than the second one, proving the former to be a better option to use. A design of a multiplexed Organ-on-chip was also made on SOLIDWORKS® 2019 with four parallelized channels that has the capability for real-time integrated TEER sensing. Flow simulation was performed to validate the channel geometry used in the design.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Programme:Biomedical Engineering MSc (66226)
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