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Surface charge of single chain nanoparticles and protein adsorption : the effect of zwitterionic ligands on the formation of a protein corona.

Sprik, P.J. (2021) Surface charge of single chain nanoparticles and protein adsorption : the effect of zwitterionic ligands on the formation of a protein corona.

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Abstract:Single chain nanoparticles are an emerging field which show a lot of promise in drug delivery. Their intramolecular folding makes them extremely small compared to classic nanoparticles. In this thesis pentafluorophenyl copolymers will be crosslinked and functionalised with a zwitterionic amine (ADPS) and histamine, reaching conversions of 7, 16 and 21\% for ADPS and 9, 20 and 23\% for histamine. The nanoparticles were functionalised with ADPS for anti-fouling purposes, but was shown to increase the protein corona compared to a nanoparticle with only aminoglycerol groups. The nanoparticles were functionalised with histamine to research their tumour targeting properties, unfortunately the yield was too low to experiment with. The uptake and cytotoxicity of nanoparticles with ADPS ligands were analysed in HeLa and RAW 264.7 cell lines. The uptake of the nanoparticles increased with a higher percentage of ADPS ligands, and the RAW 264.7 cell line saw significantly more uptake than the HeLa cell line. The metabolic activity of both cell lines was increased 24 hours after addition of nanoparticles compared to controls showing no significant cytotoxicity. This research shows that particles with zwitterionic ligands are less effective than nanoparticles with aminoglycerol ligands at the prevention of a protein corona.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:44 medicine
Programme:Biomedical Technology BSc (56226)
Link to this item:http://purl.utwente.nl/essays/86812
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