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Using FRET probes to determine state of disassembly of cowpea chlorotic mottle virus.

Elbert, Thieme (2021) Using FRET probes to determine state of disassembly of cowpea chlorotic mottle virus.

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Abstract:Many people in the world lack access to clean water and sanitation. Either it is too far away, or it is contaminated with pathogens and dirt. Many new ways to create clean water are being researched, one of them being cationic membranes, which can potentially disassemble virus capsids. A big problem in the development of new antiviral membranes is tracking their effectiveness. Virus particles are very small, and it is difficult to prove they are fully disassembled. In this report, a new method using FRET labeling to determine state of virus disassembly is researched. The FRET dyes were bound to CCMV, a model virus, which was then disassembled by adding either SDS or PEI. Using FCCS and emission spectra, the samples were analyzed. The results show this method is viable for determining state of disassembly of viruses, though more tests are needed to optimize the procedure.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:30 exact sciences in general, 33 physics, 35 chemistry, 42 biology, 50 technical science in general
Programme:Biomedical Technology BSc (56226)
Link to this item:http://purl.utwente.nl/essays/87843
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