University of Twente Student Theses


Using lung-on-a-chip model to study the differences in translocation of nanoplastics through lung epithelium

Katgert, E. (2021) Using lung-on-a-chip model to study the differences in translocation of nanoplastics through lung epithelium.

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Abstract:Plastic pollution is a problem from the modern era, studies have found that plastics can be broken down via bio and non-biodegradation into small sizes of plastics, called nanoplastics and microplastics. These plastics can travel through air and end up in the human lungs. Possible effects of presence of these plastics in the lungs are inflammation reactions, oxidative stress and immune dysfunction. In this study we will focus on nanoplastics which have proven to be able to be taken up through active and passive transport in alveolar epithelium. The aims of this research are to build a lung-on-a-chip which mimics the alveolar structure and has Collagen 1 as a foundation. The other aim is to examine whether and how nanoplastics could transfer through tubular alveolar epithelium in the established device. At last, we want to determine if nanoplastics could disturb the tight junctions of alveolar epithelium, since this has not been researched for a tubular alveolar epithelial structure. The device that is used in this research was made from polydimethylsiloxaan which is a soft elastomer after curing in a 3D printed mold. After, inlets and outlets were punched and the chip was prepared for cell seeding of Calu-3 and A549 cells and incubation of green fluorescent nanoplastics with the sizes 50 and 100 nm. The following concentrations of nanoplastics were used 10, 100 and 1000 μg/mL. Staining the nuclei, cell membrane and ZO-1 proteins resulted in determination of where the nanoplastics had traveled to in the device.
Item Type:Essay (Bachelor)
Faculty:EEMCS: Electrical Engineering, Mathematics and Computer Science
Subject:33 physics, 42 biology
Programme:Biomedical Technology BSc (56226)
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