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The effect of anthracyclines on the gene and protein expression of transcription factors IRX4 and HAND2 in cardiomyocytes

Kempers, M.H. (2021) The effect of anthracyclines on the gene and protein expression of transcription factors IRX4 and HAND2 in cardiomyocytes.

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Abstract:Anthracyclines, like Doxorubicin, are known to be cardiotoxic. This toxicity is for example caused by altered gene and protein expression which leads to apoptotic cell death. This research focuses on the effect of anthracyclines on the gene and protein expression of transcription factors IRX4 and HAND2. Aside from Doxorubicin, these effects are also research for new analogues of Doxorubicin; Amrubicin and Aclarubicin. In addition, the research also looks into the effect of cotreatment with Bortezomib on the gene and protein expressions of these transcription factors. This is evaluated by qPCR-based gene expression analysis and multiple immunostaining experiments for protein expression. These experiments showed that the transcription factor IRX4 is not affected by anthracyclines on both gene and protein level. The gene expression of HAND2 decreases after treatment with Doxorubicin and also the protein expression of HAND2 shows a decrease after treatment with Doxorubicin and Aclarubicin. The decrease of HAND2 proteins seems to increase with a higher concentration of anthracycline and with a longer treatment time. No conclusions can be drawn about the effects of Amrubicin treatment on HAND2 protein expression based on this research because of autofluorescence signal in the GFP channel caused by Amrubicin. The cotreatment with Bortezomib did not seem to have an effect on the gene and protein expression of IRX4 and does not prevent the downregulation of HAND2.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Programme:Biomedical Technology BSc (56226)
Link to this item:http://purl.utwente.nl/essays/89223
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