University of Twente Student Theses
Investigating 14-3-3’s Binding Site upon Interaction with α-Synuclein Through Microscale Thermophoresis Based Competitive Assay
Veldman, O.P. (2022) Investigating 14-3-3’s Binding Site upon Interaction with α-Synuclein Through Microscale Thermophoresis Based Competitive Assay.
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| Abstract: | Parkinson’s Disease (PD) is a neurodegenerative disease characterized by the aggregation of α-synuclein to form toxic multimers in the neuronal cells of the body and brain. It has been shown that the 14-3-3 protein family influences the aggregation of α-synuclein by binding its multimers, preventing misfolding and delaying aggregation. This insight forms opportunities for potential therapeutic approaches regarding the pathogenesis of PD. Here, we performed a competitive assay based on microscale thermophoresis and show that 14-3-3’s amphipatic binding pocket may potentially be involved in the interaction between α-synuclein multimers and 14-3-3 proteins. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 42 biology, 50 technical science in general |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/92114 |
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