University of Twente Student Theses
Effect of electrical stimulation on the contractile performance of engineered heart tissues (EHT)
Kutucu, BSc. Ceren (2022) Effect of electrical stimulation on the contractile performance of engineered heart tissues (EHT).
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| Abstract: | Three dimensional models like engineered heart tissues (EHT) have the advantage of resembling human physiology. However, they are made from human pluripotent stem cell derived cardiomyocytes (hPSC-CM), that exhibit an immature phenotype. By conditioning hPSC-CMs electromechanically, improvements in contractile performance and expression of biological markers can be observed. This research focuses on the effect of continuous electrical stimulation on the contractile properties and the biological markers of the EHTs. Previous researches have shown that ramp stimulation of EHTs has the most effect on improvements. However, they lack the determination of the most optimum electrical conditions for the survival and maturation of the CMs. A previously designed EHT platform is used to make EHTs. A mathematical model and a simulation of the EHT platform were made to identify the well resistance and current distribution by the tissues and those results were compared with the experimental setup. There was high correlation between the model and the experiment. The most suitable electric field was determined for cell survival throughout continuous stimulation. The effect of continuous stimulation was then analyzed. Results present the change in these parameters and possible causes of change were discussed. |
| Item Type: | Essay (Bachelor) |
| Faculty: | EEMCS: Electrical Engineering, Mathematics and Computer Science |
| Programme: | Electrical Engineering BSc (56953) |
| Link to this item: | https://purl.utwente.nl/essays/92301 |
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