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Parallelization of in vivo-like anti-cancer drug concentration profiles on top platform for testing drug efficacy on tumour spheroids

Nijboer, J.G.K. (2022) Parallelization of in vivo-like anti-cancer drug concentration profiles on top platform for testing drug efficacy on tumour spheroids.

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Abstract:In this project, an in vitro, microfluidic system is introduced, with which dynamic, in vivo-like, anti-cancer drug concentration profiles can be sequentially parallelized. One microfluidic chip was developed and used twice in the system. First, to generate the dynamic concentration profile, and subsequently for sequential parallelization of the concentration profiles over three microfluidic channels. Colorectal tumour spheroids were integrated in the microfluidic channels to form a Tumour-Spheroid-on-Chip model. The ability of the system to steadily parallelize a dynamic concentration profile was successfully demonstrated, using fluorescein as a model drug. Additionally, it was proven that the spheroids were able to grow on chip, during sequential parallelization of cell culture medium. Finally, three tumour spheroids were placed in series in the three sep- arate microfluidic channels. The total of nine spheroids were exposed to the in vivo oxaliplatin concentration profile, where growth inhibition was observed for all spheroids. A proposed method for further upscaling of the system was given, where the microfluidic chips can be integrated on the Translational-Organ-on-Chip platform to be able to simultaneously test up to two different in vivo anti-cancer drug concentration profiles over a total of 18 tumour spheroids.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology, 44 medicine
Programme:Biomedical Engineering MSc (66226)
Link to this item:https://purl.utwente.nl/essays/92500
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