University of Twente Student Theses


Optical Mapping of Isotonically Contracting Living Myocardial Slices: A Proof-of-Principle

Everts, B.R. (2023) Optical Mapping of Isotonically Contracting Living Myocardial Slices: A Proof-of-Principle.

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Embargo date:24 February 2024
Abstract:Introduction Cardiovascular disease (CVD) poses a great health burden. However, the underlying causes of CVD, such as disturbances in action potential (AP) generation and propagation, and excitation-contraction coupling (ECC) are poorly understood. Many in vitro methodologies are available to study cardiac electrophysiology and ECC, each having limitations. This thesis aims to provide a new experimental setup to study cardiac electro(patho-)physiology in isotonically contracting myocardial slices (LMS): motion-corrected optical mapping. Methods Based on literature, criteria for the optical mapping system were determined. The acquired optical mapping system was used to perform proof-of-principle experiments. Marker-based image registration and excitation ratiometry were used to reduce motion artefacts. Images were acquired at 2000 frames per second to record the rapid AP-upstroke. Results An optical mapping system for isotonically contracting LMS was built. Image registration was based on markers. Excitation ratiometry was achieved through TTL-pulses for light sources and camera. The first optical mapping experiments on isotonically contracting LMS were performed, combined with contractile force measurements. Conclusion This thesis provides the framework for the first optical mapping experiments on isotonically contracting LMS. Based on preliminary results, optical mapping has the potential to become invaluable in deciphering questions related to AP generation, propagation, and ECC.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:44 medicine
Programme:Technical Medicine MSc (60033)
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