University of Twente Student Theses


Mechanically activated in-vitro muscle model

Doets, M. (2022) Mechanically activated in-vitro muscle model.

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Abstract:Spasticity is a common disorder that more than 12 million people on world scale are suffering from. Within this disorder high amounts of contraction stimulus and velocity-depended stretch reflexes are seen, which result in possible shortening or stiffening of the muscle. It was aimed to design an in-vitro system to prove eccentric training as a stimulus for longitudinal muscle growth by sarcomere addition in series, to improve locomotion of patients with spasticity. This goal was aimed to be reached within 11 weeks. Requirements were formulated to control suitability for the main goal. Six systems were evaluated. A 3D printed prototype was designed and fabricated. Accuracy measurement of the system was performed for steps distances between 10 and 0.1 mm for increasing and decreasing pillar distance. For this measurement, a syringe pump was used with an accuracy of ± 0.25%. The steps were performed with a speed of 220 µm/s, the movement was tracked by a digital microscope. It was found that a hydrostatic pressure-driven system using PDMS pillars and a syringe pump was most adequate. This system fits six tissues with a maximal of 2.5 · 105 cells per tissue. It is seen that accuracy decreases by downsizing the step distance, from 86.4 % ± 0.8 for the steps of 10 mm to 54 % ± 10 for steps of 0.1 mm. The potential to mechanically stretch tissue by this system was proven by theory. It can be concluded that the system is up to nine of the eleven set requirements. The system has very high potential to be used to proof eccentric training as stimulus for longitudinal muscle growth.
Item Type:Essay (Bachelor)
Faculty:ET: Engineering Technology
Subject:42 biology, 44 medicine, 50 technical science in general, 51 materials science, 52 mechanical engineering
Programme:Biomedical Technology BSc (56226)
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