University of Twente Student Theses


Effect of phototherapy on fat-fed hepatocyte

Kroon, Evelien (2022) Effect of phototherapy on fat-fed hepatocyte.

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Abstract:Non-alcoholic steatohepatitis (NASH) is an excessive built up of fed in the liver that inhibits the liver to perform all it functions and can lead to liver cirrhose. It is also the most common liver disorder in western countries. Besides a big change in living style and diet, there is no know working treatment to heal the liver. In recent years, research has been done to see if photobiomodulation (PBM) could be used for treatment of the liver. PBM is a non-invasive, non-thermal light therapy that uses visible and near-infra red (NIR) light to stimulate the cells so that they can reduce inflammation, accelerate wound repair, restore the body’s homeostatic balance and help with neurological pain and pathologies. In this research, two iCAP devices were used, one with LED’s with a wavelength of 660 nm (red) and one with a wavelength of 810 nm (near infra-red or NIR). The HepaRG, primary human hepatocytes, cells were treated with a combination of oleic and palmitic acid, isopropanol control and control treatment which was left on the cells for 24 hours. When the treatments were added, the iCAP device was placed on the 12 well plates for 30 minutes. To analyse the working of the iCAP device multiple methods were used, namely: oil red o, Hoechst, TMRE and DCFDA staining and an alamar blue assay. When analysing the results, it shows no effect of the 660 nm wavelength LED’s but some positive effect of the 810 nm wavelength device. Further research to use 660 nm for treatment of NASH is not advised, but for 810 nm further research is necessary to see the extent of the effect on the cells.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:42 biology, 44 medicine
Programme:Biomedical Technology BSc (56226)
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