University of Twente Student Theses
Implementation of proximity ligation assay to quantify low protein concentrations in low volume samples
Steenbergen, J.J. van (2022) Implementation of proximity ligation assay to quantify low protein concentrations in low volume samples.
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| Abstract: | Vessels-on-a-chip are used to mimic the blood vessels and find the influence of the substances on the endothelial cells covering the inside. Several studies have shown that inflammatory cytokines in blood plasma can disrupt these endothelial cells. A different student at AST used ELISA to quantify the concentration of these cytokines with ELISA but one cytokine, IL-1β could not be measured due to its low concentration, therefore another method, SP-PLA, is set up. This method is able to quantify low concentration and low volume samples. When implemented successfully this method could later be used to quantify a broader range of proteins. PLA probes were used to label the IL-1β antigen with oligonucleotides that were amplified with qPCR. A standard series was made to check the overall fitness of the SP-PLA. The qPCR data showed that the basics of the SP-PLA are working but it still has to be improved. The standard deviations of the standard series are low but a significant calibration curve could not be constructed. IL-1β samples could not yet be measured, some further research is necessary to make the SP-PLA operable. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 35 chemistry, 42 biology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/94825 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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