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The effect of histamine and cetirizine on endothelial cells : The effect of histamine and its antagonist cetirizine on the permeability of a monolayer of Human Umbilical Vein Endothelial Cells (HUVECs)

Endhoven, Krissie (2022) The effect of histamine and cetirizine on endothelial cells : The effect of histamine and its antagonist cetirizine on the permeability of a monolayer of Human Umbilical Vein Endothelial Cells (HUVECs).

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Abstract:Age-related Macular Degeneration is a progressive eye disease that affects millions of people worldwide. It is distinguishable between wet and dry AMD but are both initiated by an inflammatory cascade reaction. There are a couple of known treatments but they are primarily for late stage AMD. To simulate the inflammatory state of the endothelium in the disease, Human Umbilical Vein Endothelial Cells (HUVEC) were incubated with an antihistamine drug cetirizine, an aspired treatment drug for early stage AMD, and its antagonist histamine, a potent inflammatory mediator. The HUVECs were incubated until confluent and after incubation of either histamine, cetirizine or histamine and cetirizine, were stained with an immunostaining for VE cadherin, a molecule that composes the tight junctions between cells. A transwell permeability assay was also performed under similar conditions. There was a noticeable difference in cell morphology upon a concentration of 100 μM histamine and 100 μg/mL cetirizine incubation, the 100 μg/mL cetirizine also seemed toxic for the HUVECs monolayer. The 10 μM histamine and 10 μg/mL cetirizine concentration did not alter the morphology conclusively. Further on, the 10 μg/mL cetirizine concentration did not influence the monolayer permeability induced by a 100 μM histamine concentration. Future experiments should focus on permeability assays like the transwell experiment or a trans-endothelial electrical resistance (TER) measurement.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:44 medicine
Programme:Biomedical Technology BSc (56226)
Link to this item:https://purl.utwente.nl/essays/94910
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