University of Twente Student Theses


Laser Doppler Flowmetry, using in vitro phantoms to study skin microcirculation

Lookeren Campagne, M.J.A. van (2023) Laser Doppler Flowmetry, using in vitro phantoms to study skin microcirculation.

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Embargo date:17 May 2024
Abstract:Laser Doppler flowmetry (LDF) is a technique to measure perfusion in the skin. It uses the optical Doppler effect to measure the movement of bl This research has taken a first step towards understanding the composition of laser Doppler flowmetry signals. In vivo LDF measurements were performed to gain experience with the system and to observe differences in the signal. The measurements showed a unique cardiac pattern in the perfusion signal. SDF was done to visualise the microcirculation of blood within the skin. As a first step in the simulation of microcirculation, phantoms were made with a 100 µm diameter channel. To examine the shape of the channels in the phantoms, brightfield and fluorescence microscopy has been performed. Then, a blood flow was realised inside the channels. An LDPM measurement was performed on this blood flow. Although a signal was obtained, no relationship between flow rate and perfusion could be established. In order to enable a comparison between the in vitro and in vivo measurements, systematic measurements should be carried out where the flow rate in the channel can be both controlled and optically measured.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:42 biology
Programme:Biomedical Technology BSc (56226)
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