University of Twente Student Theses


Designing zero-mode waveguides for fluorescence experiments on α-synuclein and DNA

Loenhout, F.H.C. van (2022) Designing zero-mode waveguides for fluorescence experiments on α-synuclein and DNA.

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Abstract:Zero-mode waveguides (ZMW) are a mechanical way to lower the detection volume in fluorescence detection methods like Fluorescence Correlation Spectroscopy (FCS) and Two Colour Coincident Detection (TCCD) experiments. The decrease of detection volume allows for single molecule detection experiments at concentrations up to a thousand times higher than the ones accessible by conventional methods. Apart from restricting the detection volume, these waveguides are also reported to enhance fluorescence signal and quench fluorophore lifetime. These properties make ZMWs promising assets when investigating molecular dynamics or aggregation, for instance in the ongoing research into alpha-synuclein. This thesis will design, fabricate and test ZMW to assess their suitability to probe biomolecular interactions. To that end, the performance of the ZMW in terms of signal enhancement, lifetime quenching and restriction of the observation volume is evaluated. After establishing the functionality, interaction experiments with alpha-synuclein and DNA are performed.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:33 physics, 42 biology, 51 materials science, 52 mechanical engineering
Programme:Biomedical Engineering MSc (66226)
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