University of Twente Student Theses
The effect of charged single chain polymeric nanoparticles on alpha-synuclein aggregation in SH-SY5Y cells
Buijs, F.A.H. (2023) The effect of charged single chain polymeric nanoparticles on alpha-synuclein aggregation in SH-SY5Y cells.
|
PDF
5MB |
| Abstract: | Parkinson’s disease (PD) is an increasing problem, with more than a million cases worldwide. 95% of the cases is sporadic and to decrease the amount of cases, it is important to find what can induce PD. A characteristic of the disease is the aggregation of α-synuclein, causing dopaminergic neurons to die. The aggregation can be caused by positive particles, next to that nanopolymers, produced for controlled drug delivery, with tertiary amine can enter the cytosol where they can reach the α-synuclein. The goal of this research is to find out if these nanopolymers can induce α-synuclein aggregation, in and outside the cell. For this the affinity is researched, just like the aggregation outside the cell and the effect of the nanoparticles on α-synuclein in SH-SY5Y cells. It is shown that nanoparticles with 60% tertiary amine (positively charged) can induce aggregation. The higher the amount of tertiary amine, the faster aggregation occurs and the more toxic the nanoparticles are. Nanoparticles with 0% tertiary amine (negatively charged) limit the aggregation. In SH-SY5Y cells, none of the nanoparticles induce aggregation in 72 hours. This research shows that the nanopolymers can be toxic to the brain and must therefore be handled with care. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 30 exact sciences in general, 35 chemistry, 42 biology, 44 medicine |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/95746 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
Show download statistics for this publication
Show download statistics for this publicationRepository Staff Only: item control page