University of Twente Student Theses


Short-term effects of electrical stimuli on engineered skeletal muscle tissue growth

Oude Voshaar, Maartje (2023) Short-term effects of electrical stimuli on engineered skeletal muscle tissue growth.

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Abstract:Muscle weakness is a common consequence of advanced disease, which can have a great impact on the patient’s quality of life. The utilization of electrical stimulation in tissue engineering has shown promising outcomes in promoting skeletal muscle tissue growth and maturation. In this study, the growth characteristics of three-dimensional mouse C2C12 skeletal muscle tissue in response to electrical stimulation were researched. Electrical stimulation was performed using electrodes. The growth characteristics were assessed by evaluating the tissue structure and compaction in response to electrical stimulation. The parameters used to measure structural changes in the skeletal muscle tissue, namely spheroid roundness and circularity, indicated no change in tissue structure over time as a result of electrical stimulation. Electrical stimulation did result in a different projection area regression model than the control group, linear and second order polynomial regression, respectively. A higher stimulation amplitude did, however, not result in a significantly different slope when comparing the 4V and 8V projection area data. Due to spheroid adherence to the well surface and the absence of two adherence surfaces necessary for realistic mechanical stimulation, more research is needed in order to evaluate the effect of electrical stimulation on tissue growth and structural characteristics. Mechanical stimulation using two adhesion points, and evaluating tissue differentiation factors by means of qPCR analysis, is recommended for additional research.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Programme:Biomedical Technology BSc (56226)
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