University of Twente Student Theses


Development of a Ligament-on-Chip Aligning PDL-hTERT cells on a PDMS membrane

Toet, M.J. (2023) Development of a Ligament-on-Chip Aligning PDL-hTERT cells on a PDMS membrane.

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Embargo date:31 July 2027
Abstract:The ligament provides stabilization for example the knee joint when a force is applied on the knee. It consists of an aligned collagen matrix with aligned cells on top. Besides, the ligament is involved in several diseases, like injuries, inflammation and arthritis. Several studies are about the ligament to study the mechanisms of these diseases, but these studies lack the combination of the collagen matrix, aligned cells or mechanical stimulation of the cells. When modeling diseases on these limited tissues, results will not be accurate. Organon- chip technology can maybe provide the combinations of all the aspects of a ligament and replicate more accurately the in vivo ligament. Therefore, this research investigated the development of a ligament-on-chip by aligning PDL-hTERT cells on a PDMS membrane. First of all, the effect of fluid flow shear stress on the alignment of PDL-hTERT cells in chips was investigated. The shear stress was here 0-1 dynes/cm2. The cells were only aligned around the inlet of the chip and had an orientation of -45 and 45 degrees. Secondly, the alignment of collagen by flow was investigated. Some collagen was aligned, but most collagen was accumulated or had a random distribution. Lastly, mechanical actuation was performed on manually aligned assemblies. Most assemblies were not well aligned and the success rate was not significant. Mechanical actuation worked only for the well-aligned assemblies. Two chip designs were compared. Design 2 with sideboxes and boxes on top showed deformation in the middle of the membrane. Design 1 showed no deformation in the middle of the membrane. The three experiments need to be improved and combined in the future, to completely mimic the ligament.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:33 physics, 35 chemistry, 42 biology
Programme:Biomedical Technology BSc (56226)
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