University of Twente Student Theses
Hoffas’s Fat Pad-on-Chip : Optimization and Characterization of Dextran- Hyaluronic Acid - Gelatin Hydrogels to Mimic the HFP Extracellular Matrix
Scheffer, Lars (2023) Hoffas’s Fat Pad-on-Chip : Optimization and Characterization of Dextran- Hyaluronic Acid - Gelatin Hydrogels to Mimic the HFP Extracellular Matrix.
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| Abstract: | Hoffa’s fat pad (HFP) is an articular fat pad located inside the knee joint. Recently more research has been done into the role of HFP as an inflammatory regulator. In this bachelor assignment, hybrid hydrogels consisting of hyaluronic acid-tyramine (HA-TA), dextran-tyramine (Dex-TA), and gelatin-tyramine (Gel-TA) will be optimized and characterized. In this proof-of-concept experiment the most promising hydrogel for developing a Hoffa’s fat pad-on-chip (HFPoC) will be determined. To find the right hydrogel, multiple experiments were performed with the hybrid hydrogel at 1.25%, 2.5%, and 5% wt concentration. Firstly, rheology was carried out to find which hydrogel mimics the HFP ectracellur matrix stiffness the closest. Since mechanical actuation is a crucial part of an HFPoC, an on-chip experiment was then set up to examine the effect of actuation on the different hydrogel concentrations. In this experiment, chips were injected with fluorescent beads-infused hydrogels. The added beads allow for tracking of the mechanical actuation and determining the displacement using fluorescence. Finally, the hydrogels were tested for their biocompatibility in a 3D cell-culture experiment. Chips and 96-well plates were seeded with PDL-hTERT cells encapsulated in the hydrogels. Live/dead stainings were performed at different time points during the culture period. The rheology results showed that the stiffness of the 1.25% hydrogel was closest to that of human adipose ECM. The 1.25% hydrogel also allowed for the most displacement of the beads. It was found that in all hydrogel concentrations, the beads displacement increased over time. The cell culture experiments showed cell stretching and migration in the 1.25% hydrogel. The 2.5% showed minor cell stretching, and there was no stretching in the 5% hydrogel. These experiments indicate that out of the three hydrogel concentrations tested, the 1.25% hydrogel consisting of HA-TA, Dex-TA, and Gel-TA could be used as a scaffold when creating an HFPoC. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 30 exact sciences in general, 42 biology, 51 materials science |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/96451 |
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