University of Twente Student Theses
Effect of cycles of electrical stimulation on the contractile performance of engineered heart tissue using human-induced pluripotent stem cell-derived cardiomyocytes
Halmingh, Q. (2023) Effect of cycles of electrical stimulation on the contractile performance of engineered heart tissue using human-induced pluripotent stem cell-derived cardiomyocytes.
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| Abstract: | Engineered Heart Tissue (EHT) is a three-dimensional in vitro model that can resemble the cell-cell interaction and tissue organization of the human heart. This in vitro model can be patient-specific by using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs); however, hiPSC-CMs have shown an immature phenotype which does not resemble the in vivo situation. By introducing electromechanical stimulation, the maturation phenotype can be improved, and as a result, higher contractile performance of the hiPSC-CMs can be observed. The optimal electromechanical conditions to improve maturation have not been determined. Therefore, this research focuses on evaluating the effect of different cycles of electrical stimulation on the contractile performance of hiPSC-CMs. Multiple stimulation cycles were tested over a period of five days. The effect induced was evaluated on the physiological level by the change in the force of contraction and spontaneous frequency as well as electrically by the excitation threshold, and current and resistance distribution. This research opened the door to cycles of electrical stimulation on EHTs but further research is necessary to investigate if cycles of electrical stimulation have the potential to improve hiPSC-CMs maturation levels. |
| Item Type: | Essay (Bachelor) |
| Faculty: | EEMCS: Electrical Engineering, Mathematics and Computer Science |
| Subject: | 53 electrotechnology |
| Programme: | Electrical Engineering BSc (56953) |
| Link to this item: | https://purl.utwente.nl/essays/96466 |
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