University of Twente Student Theses
Testing the applicability of the Omi perfusion device for research into circulating tumour cells : Research into the possibility of circulating tumour cells and the effect of flow on cell culture on chip models using the Omi perfusion device.
Tolsma, C.S. (2023) Testing the applicability of the Omi perfusion device for research into circulating tumour cells : Research into the possibility of circulating tumour cells and the effect of flow on cell culture on chip models using the Omi perfusion device.
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| Abstract: | In this study the Omi automated perfusion system by Fluigent will be beta-tested for use in cancer research. Omi is designed to be able to recirculate culture medium over organs-on-chip, using small amounts of fluid. Two experiments were designed to test the suitability of Omi. The first experiment had Omi recirculate cells to determine the effect of circulation on the viability of the perfused cells. Aside from that Omi components were analysed for aggregation of cells after circulation. The second experiment had Omi recirculate medium over blood vessel-on-chips to investigate the effect of flow on the cells. The results concluded that circulation of cells did not impact the viability, but lowered the concentration. Omi components showed no evident aggregation of cells. Furthermore, flow caused endothelial cells seeded at a low density to align in the direction of the flow. At a high seeding density cells did not show this behaviour. The first experiment could only be performed once and should be repeated to establish a more reliable view of the cell circulating capabilities of Omi. If these tests are complete Omi could prove useful for research into the effects of circulating tumour cells on blood vessel on microfluidic chip models. |
| Item Type: | Essay (Bachelor) |
| Clients: | Fluigent, Le Kremlin-Bicêtre, France |
| Faculty: | EEMCS: Electrical Engineering, Mathematics and Computer Science |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/96534 |
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