University of Twente Student Theses
A metabolic support system for engineered (micro)tissues using glycogen-releasing cell-adhesive micromaterials
Porsul, I.R. (2023) A metabolic support system for engineered (micro)tissues using glycogen-releasing cell-adhesive micromaterials.
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| Abstract: | Tissue engineering aims at the creation of living implants to replace, repair, or regenerate damaged, diseased, or aged tissues. However, the current methods to create engineered tissues are limited to either small or thin tissues and creating clinically relevant sized tissues remains one of the major challenges in the field of tissue engineering. These larger tissues are characterized by limited oxygen and nutrient diffusion during the prevascular phase, leading to the formation of a necrotic core within days. This prevascular phase can be bridged by continuously supplying the incorporated cells with nutrients. Glycogen is a molecule that is present in the human body, and functions as a storage and release system of glucose molecules. It was already known that glycogen is present inside cells, but we show that cells can survive with the supply of solely exogenous glycogen. To use glycogen as a controlled release system of glucose molecules, we incorporated it into liquid core shell microgels. We showed that upon culture of human mesenchymal stem cells (hMSCs) with glycogen loaded core shell microgels, a high cell viability and metabolic activity can be maintained not only in 2D culture but also in 3D utilizing collagen hydrogels. The data obtained thus far have demonstrated promising and further research will be performed to investigate the potential of glycogen-loaded core shell microgels to fully bridge the prevascular phase of tissue engineered constructs. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 35 chemistry, 42 biology, 44 medicine |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/96588 |
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