University of Twente Student Theses


3D-printed monolithic oviduct-on-chip device

Bertelink, S.H. (2023) 3D-printed monolithic oviduct-on-chip device.

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Embargo date:11 July 2028
Abstract:Infertility is a rising problem. As 1 in 10 persons of childbearing age are infertile, use of assisted reproductive technologies (ART) is steadily increasing. Yet, success rates remain low and recent studies suggest that the procedure may be harmful to the gametes and resulting embryos. Of note, in vivo, gametes and embryos are continuously communicating with the oviduct epithelium, which is not the case in ART procedures. In this context, an organ-on-chip model of the oviduct including a functional epithelium can provide an environment closer to the in-vivo situation. Previously, we developed an oviduct-on-chip which, compared to conventional Petri dishes, incorporated dynamic flow, and adequate shear on all cells. This model consisted of two compartments, separated by a porous membrane onto which primary oviduct epithelial cells were grown. The bottom channel provides pillars functioning as a trapping structure for oocytes/embryos. In this work, we propose a new design with a dam structure, to reduce shear on the oocytes and embryos, as well as an innovative fabrication process, using 3D printing to produce a monolithic oviduct-on-chip platform. We have compared various post-processing of the 3D-printed materials to ensure cytocompatibility, as assessed using monocyte cell lines, gametes and primary oviduct cells.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology, 44 medicine, 51 materials science, 52 mechanical engineering
Programme:Biomedical Engineering MSc (66226)
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