University of Twente Student Theses
White blood cell depletion of immunomagnetically enriched samples using antibody functionalised surfaces
Duijkers, Floortje (2023) White blood cell depletion of immunomagnetically enriched samples using antibody functionalised surfaces.
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| Abstract: | CTCs are rare cells that are present in the blood of metastatic cancer patients. To increase the number of CTCs that can be obtained from a patient to enable efficient characterisation of the tumor status and ultimately guide therapy, a large amount of the patient’s blood needs to be screened. DLA is a method to increase the volume of blood that can be screened. However, current CTC enrichment techniques such as CellSearch encounter the problem that too much unwanted WBCs are captured along CTCs, which hinders the screening of large DLA volumes and subsequent sample analysis. A way to overcome this problem is to deplete the WBC from the previously enriched sample. As CellSearch makes use of immunomagnetic particles to enrich CTCs, it was attempted to develop and test a WBC depletion technique that can deplete immunomagnetically enriched samples. This technique makes use of antibody-coated surfaces with antiCD45-antibodies that are targeted specifically at WBCs and not CTCs. Out of the two tested surface types, the coated glass surface performed best because less non-specific binding of TCs was observed compared to an antibody-coated hydrogel layer. Further testing is needed to assess the cell recovery in the depleted sample. Nonetheless, the presented technique shows potential to enable the screening of large blood volumes of cancer patients through WBC depletion of immunomagnetically enriched samples. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 42 biology, 44 medicine, 50 technical science in general |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/97014 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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