University of Twente Student Theses

Login
As of Friday, 8 August 2025, the current Student Theses repository is no longer available for thesis uploads. A new Student Theses repository will be available starting Friday, 15 August 2025.

THP1 model of Classical monocytes for the study of Non-alcoholic fatty liver disease

Groenenboom, A.R. (2023) THP1 model of Classical monocytes for the study of Non-alcoholic fatty liver disease.

[img] PDF
8MB
Abstract:Monocyte-like cell lines are invaluable as models for monocytes in research regarding natural biological states as well as in the context of pathology, treatment target discovery and drug testing. However, reports regarding findings relating monocyte-like cells are regularly contradictory due to the plasticity of monocytes and their sensitivity to culture conditions. In addition, two prominently used models, THP1 and U937, are naturally at the preceding stage of promonocytes. Hence, differentiation of the cell lines to a mature profile is required for use in research. However, maturation and differentiation of monocytes is still poorly understood, and as a result no replicable protocol for THP1 and/or U937 cell lines has yet been established. This research investigates the effect of a number of maturation protocols, and potentially influential culture specifics that are prone to vary between labs, on the maturity profile of promonocytes through flow cytometry of expression levels of CD14, CD16, CD13 and HLA-DR.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology, 44 medicine
Programme:Biomedical Engineering MSc (66226)
Link to this item:https://purl.utwente.nl/essays/97559
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page