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The influence of viscoelastic properties and surface roughnesses in an alginate-based hydrogel on the behaviour of hiPSC-CMs

Heer, Inge de (2023) The influence of viscoelastic properties and surface roughnesses in an alginate-based hydrogel on the behaviour of hiPSC-CMs.

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Abstract:The extracellular matrix (ECM) significantly influences the behaviour of cells by mechanical and biochemical cues. An understanding of the role of these cues is necessary to create a suitable threedimensional (3D) in vitro model that mimics the ECM of the native tissue, enabling cells to attach, spread and proliferate. Hydrogels are a class of biomaterials suitable for this application due to their high water content and biocompatibility. Here, a covalently crosslinked alginate-based hydrogel including RGD sequences was prepared and seeded with human-induced pluripotent stem cells derived from cardiomyocytes (hiPSC-CMs). Surface roughnesses were imparted by 3D printing a pattern onto the mould of the hydrogel. Since the covalently crosslinked hydrogel results in an elastic substrate and the embryonic cardiac tissue contains viscoelastic properties and exhibits stress relaxation, dynamic noncovalent bonds using cucurbit[8]uril and FGGC peptides were included. The storage modulus (G’), the loss modulus (G”) and the stress relaxation were quantified and the influence of surface roughnesses was examined on the behaviour of the hiPSC-CMs. The results from this study demonstrate the role of the covalent crosslinking of the hydrogels and the presence of the dynamic bonds.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:35 chemistry, 42 biology, 51 materials science
Programme:Biomedical Technology BSc (56226)
Link to this item:https://purl.utwente.nl/essays/97654
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