University of Twente Student Theses
Deuterium NMR and Intracellular Tumour pH Measurement
Wijland, Casper van (2023) Deuterium NMR and Intracellular Tumour pH Measurement.
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| Abstract: | The pH profile of the environment around cancer sites can provide information about factors such as metabolism. Using NMR, information about a sample can be obtained from its magnetic resonance properties. These properties may shift with pH changes, and for this reason 1H NMR has the potential to be a useful tool as it can give pH information non-invasively. However, since physiological compounds are abundant in 1H, this technique suffers from high background. Using deuterium probes instead of hydrogen could provide more specific measurements. Histidine and imidazole were chosen as probes and their ring carbon protons were exchanged with deuterium. These deuterated probes were shown to have shifts in their 2H NMR spectra in response to pH changes, and could be good pH probes in tumours. Histidine also offers the possibility of inclusion in peptides, making it possible to tune the properties of the probe to the specific purpose of binding with the cells. A peptide synthesis was attempted but it was unsuccessful. Cell incubations were performed but no probe signal could be measured. More research is needed on the toxicity of the probe molecules and their cell interactions. The possibilities of creating a purpose-built peptide also warrant more research. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 35 chemistry |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/97692 |
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