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De invloed van normoxia en hypoxia op de adipogene, osteogene en chondrogene differentiatie van humane mesenchymale stamcellen

Baars, N.H.M. (2023) De invloed van normoxia en hypoxia op de adipogene, osteogene en chondrogene differentiatie van humane mesenchymale stamcellen.

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Abstract:When cartilage is damaged or worn out, it is quite difficult to repair. There are techniques to repair it, but those are too invasive. That is why new, less invasive techniques should be developed to restore cartilage. This research is looking for the optimal differentiation conditions in terms of oxygen tension. A low oxygen tension of 2.5% O2 (hypoxia) would result in better chondrogenesis. To see how hypoxia affects de differentiation of hMSC’s compared to normoxia (20% O2), hMSC’s were cultured in both normoxia and hypoxia conditions. Then, after 10 days of culturing, 2D osteogenic, 2D adipogenic and 3D chondrogenic differentiation were examined under both normoxia and hypoxia conditions. After 21 days of differentiation, the different cells were fixed in formalin. The cells for adipogenic and osteogenic differentiation were stained with Oil Red O and Alizarin Red S, respectively. The chondrogenic pellets were embedded in paraffin and stained with Alcian Blue and Safranin O. After this, the staining and morphology of all cells and pellets were analyzed. The results show that no osteogenic differentiation took place. Furthermore, it appears that hMSC’s that were cultured in hypoxia and differentiated in normoxia yield the best results for both adipogenic and chondrogenic differentiation.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:42 biology
Programme:Biomedical Technology BSc (56226)
Link to this item:https://purl.utwente.nl/essays/97730
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