University of Twente Student Theses
Structure-property correlations of hybrid networks from Poly(trimethylene carbonate-co-ϵ-caprolactone) and human recombinant collagen
Warmink, Lucas (2023) Structure-property correlations of hybrid networks from Poly(trimethylene carbonate-co-ϵ-caprolactone) and human recombinant collagen.
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| Abstract: | This study gave the structural-property correlations of the hybrid networks made with P(TMC-co-ϵ- CL) and human recombinant collagen. To make to functionalized copolymer, the oligomer P(TMCco- ϵ-CL) was polymerized with 1,1,1 methylol propane, resulting in a 3-armed molecule with alcohol groups at each end. Methacrylic anhydride was used for the functionalization. With the use of 1HNMR the molecular weight turned out to be 21000 g/mol and the degree of functionalization was 99%. To make the functionalized Ecol, the collagen was dissolved in PBS and stirred for several days. Methacrylic anhydride was also used for the functionalization. After adding the MA, the collagen was dialyzed against demi water for 4 more days and freeze-dried afterward for 6 more days. 1H-NMR with acetic acid D4 was used to show the characteristics of functionalization. After both the copolymer and collagen were properly functionalized, they were separately dissolved in DMSO pH2 overnight. 4 different networks (collagen, 3:5, 3:15, and copolymer) were prepared in duplo. Irgacure 2959 was used as a photoinitiator and the samples were eventually photo crosslinked with irradiation of 365nm at −15◦C under nitrogen for 1 hour. Images of the networks and SEM images were taken after network formation. The networks showed high gel contents between 89.3% and 99.7%, increasing water uptake when more collagen was present in the network and high porosity ranging between 77.1% and 84.1%. Ratios were determined using FTIR, which didn’t differ a lot from the intended ratios. Thermal properties were gathered using DSC and TGA, showing similar Tg values and an increase in decomposition temperature up till the 3:15 network. Mechanical properties were gathered with the use of DMA, which provided the E-Modulus, σ , εbreak, Toughness, SRS and Toughnesssrs. The toughness and Toughnesssrs showed an increasing trend and were significantly high for the 3:15 ratios. Overall a lot of structural property correlations were successfully determined in this study. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Subject: | 30 exact sciences in general, 35 chemistry |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/97740 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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